PCTAIRE Polyclonal Antibody

Among them, mRNA is one of the least investigated species . Until now, only a few circulating mRNA markers have been reported. These studies have revealed that circulating mRNAs can be potential biomarkers. Cyclin-dependent kinase 16 is an atypical PCTAIRE kinase, and its activity is dependent on the Cyclin Y family. Ccnys have been reported to regulate mammary stem cell activity and mammary gland development, and CCNY has been recognized as an oncoprotein in various cancers,  including breast cancer.
Our full services include protein gene synthesis, protein codon optimization, protein expression  vector design, large scale cell culture and fermentation, protein purification, and protein quality control testing. We offer significant cost saving advantages and record speed in protein expression and bulk production. We also have extensive experiences and expertise in handling various types of recombinant PCTAIRE Antibodies protein purification projects, with or without purification tags. In summary, we demonstrated that cyclin A2 is a specific activator of PCTK3 and that phosphorylation at Ser12 of PCTK3 significantly enhances its kinase activity in the absence of cyclin A2. These findings shed light on the activation mechanism of not only PCTK3 but also other uncharacterized members of the CDK family, such as PCTK2.

Avantor® can help equip your life sciences lab with the products, equipment, and supplies you need – whether you work in cell biology, genomics, proteomics, or other fields. ELISA Protocol Troubleshooting and FAQs Antibody FAQs Storage Upon receipt, store at -20°C or -80°C. Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from  different purchasing way or location, please kindly consult your local distributors for specific delivery time. When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.
Abcepta validation program described in the recent antibody quality review published in the May 2015 issue of Nature. Diagram of the study design, clinical sample flow, and process of candidate gene evaluation. The aim of this study was to identify circulating mRNA markers of NSCLC. The study design included a discovery phase and a validation phase. The whole experimental strategy is simplified in a flow chart in Figure 1.

For 3D-soft agar colony formation assay, 6-well plates were precoated with growth medium containing 1% low melting agarose (catalog no. A600015; Sangon Biotech). Cells were suspended in growth medium with 0.4% agarose and placed onto the bottom layer in triplicate (20,000 ~ 50,000 cells/well). Cells were cultured for about 4 weeks with several drops of fresh medium added every 4 days.
Immunoprecipitated proteins were then detected by immunoblotting using anti-Sec23Ap or anti-PCTAIRE-1/3 antibodies . The crystal structures of monomeric CDK16 represent the first structures from the PCTAIRE family of CDK kinases . The kinase domain exhibits the classical bilobal architecture intermixed with short insertions that help to define the CDK family fold. The complex with indirubin E804 displays some disorder in the kinase N-lobe, consistent with its requirement for a cyclin partner.

Receiver operating characteristic curve analysis of the diagnostic efficacy of plasma PCTK1 RNA levels. CDK16 inhibition is involved in multiple cancer-related signaling pathways. A KEGG analysis of the significantly decreased pathways in CDK16-KD MDA-MB-231 cells. B Network and key genes of the representative pathways enriched in the KEGG analysis. For 2D-plate colony formation assay, cells were seeded in triplicate in 6-well plates (500 ~ 1000 cells/well) and cultured for about 2 weeks.
Together these data suggest that PCTAIRE-1 can regulate protein trafficking through the secretory pathway, at least in part at the level of ER export. Two-hybrid screening was performed using the Matchmaker III system (Clontech/BD Biosciences, Oxford, UK). An adult human brain two-hybrid library, generated by both poly A and random priming, was screened using full-length human Sec23Ap as bait as previously described (Watson et al., 2005). Putative positive clones were isolated and the interaction verified by re-transformation. Clones that activated all four reporter genes in the system were identified by DNA sequencing .
The work in JP’s lab is supported by Byrd Institute Small Grant Program. Neurons were fed every third day and grown for 5 days prior to treatment. Neurons grown on 100mm dishes were treated with either DMSO vehicle or 5μM Aβ42 and harvested after 24 hrs. Neurons grown on 8-chamber were treated with wither DMSO vehicle or incremental concentrations of Aβ42 ranging from 1μM to 5μM and used for immunostaining analysis. Matsuoka S., Edwards M. C., Bai C., Parker S., Zhang P., Baldini A., Harper J. W., Elledge S.

Cells were transfected with plasmids to express GFP-PCTAIRE-1 . The K184R ('kinase-dead') point mutation in PCTAIRE-1 results in fragmentation of the Golgi and redistribution of ERGIC-53 to a more dispersed localization. In contrast, the wild type or `active' mutants of PCTAIRE-3 do not cause these effects.
A weak association was also seen with Sec24Dp but not with lamin or Sec13p. The weakly detectable interaction with Sec31Ap in plate growth assays was not seen in complementary colorimetric assays and therefore was deemed a false positive. The two-hybrid clone encoding PCTAIRE-3 includes nine amino acids from predicted intron c and 110 amino acids from predicted intron h. Using PCR, we generated a `fully-spliced' cDNA including exons 4-8, which is equivalent to the central region of the predominant isoform of PCTAIRE-3, PCTAIRE-3a (Herskovits and Davies, 2004; Okuda et al., 1992). The amino acids encoded by predicted intron h are believed to arise from incomplete splicing as they include a stop codon after 61 amino acids. The nine amino acids included in this clone would encode a known splice form, PCTAIRE-3b .

PCTAIRE kinases are almost twice the size of other members of the family because of NH2- and COOH-terminal extensions. Some members of the cdk family are predominantly expressed in terminally differentiated cells , including PCTAIRE-2 . To investigate the role played by PCTAIRE-1 in the cell division cycle, we began by analyzing the kinase’s pattern of protein expression. The data obtained indicated that contrary to PCTAIRE-2 , PCTAIRE-1 is expressed across the entire panel of cell lines that we have examined.
The possible interaction with cyclins was examined by a variety of means. First, pull-down studies were performed on the incubation of affinity-purified GST-PCTAIRE-1 with extracts of exponentially growing Hs68 cells. The result obtained showed no interaction between PCTAIRE-1 kinase and cyclin D1, E, A, B1, or B2 . Second, in vivo studies were performed by immunoprecipitating ectopically expressed HA-tagged PCTAIRE-1 from HeLa cells or endogenous PCTAIRE-1 from Hs68 fibroblasts synchronized in S phase .
Nuclear  localization of vertebrate cyclin A correlates with its ability to form complexes with cdk catalytic subunits. Analysis of substrate specificity and cyclin Y binding of PCTAIRE-1 kinase. Mechanism of CDK activation revealed by the structure of a cyclin A-CDK2 complex. Cyclin-dependent kinase 16/PCTAIRE kinase 1 is activated by cyclin Y and is essential for spermatogenesis. Two cyclin-dependent kinase pathways are essential for polarized trafficking of presynaptic components.