PRDX2 Antibody

Therefore, identification of new small-molecule inhibitors of Prdx may provide new targets and candidates for cancer treatment. Recent studies revealed that the PI3K/AKT pathway plays an important role in chemoresistance . NPC cell lines stably overexpressing miR-3188 exhibited significantly increased sensitivity to 5-FU by inactivating the /AKT pathway . NECTIN-4 increased the resistance of colon cancer cells to 5-FU by inducing the PI3K/AKT cascade . Activation of the PI3K/AKT pathway contributes to resistance to multiple cancer therapies, and is deemed a poor prognostic factor for cancers . In our study, we found that p-AKT protein levels were lower in PRDX2-depleted colon cancer cells.
In some experiments, cells were pretreated with 5 mM NAC for 1 h. Cells were grown with 50 g/ml kanamycin at 37 °C to an absorbance of 0.6 at 600 nm. Expression was induced with 0.25 mM Isopropyl-D-1-thiogalactopyranoside genways PRDX2 antibody for 12 h at 20 °C. Cell pellets were prepared and suspended in lysis buffer (50 mM NaH2PO4 and 0.3 M NaCl, pH 8.0) and disrupted by high pressure homogenizer.

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Pylori induces PRDX2 expression, protecting gastric mucosa against reactive oxygen species, oxidative DNA damage and DNA double-strand breaks. We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet. These standard curves of E2925Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

It was found that PRDX2 knockdown augmented H2O2-induced cell death in SMMC-7721 cells, whereas PRDX2 overexpression exhibited opposite effects. By contrast, PRDX2 knockdown enhanced TNF-α-induced apoptosis, whereas PRDX2 overexpression reduced it, even though both treatments showed little effects on TNF-α-induced necrosis in SMMC-7721 cells. Further exploration confirmed PRDX2 knockdown led to enhanced ROS generation in response to H2O2. Taken together, the present study supports that PRDX2 serves a pro-tumorigenic role in HCC through, at least partially, limiting ROS-mediated apoptosis under oxidative stress. Helicobacter pylori (H. pylori) infection is the main risk factor for gastric cancer. The role of antioxidant enzyme peroxiredoxin 2 in gastric tumorigenesis remains unknown.
On the other hand, the sensitivity of this test would be not high enough to be used for screening purposes in general or high risk populations. Other potential autoantibody markers identified in the present study ] that could enhance the sensitivity of our present combined assay. All human colon carcinoma tissues were collected from colon cancer patients during surgery at the First Affiliated Hospital of Chongqing Medical University . The study was approved by the Ethics Committee of the First Affiliated Hospital of Chongqing Medical University . Informed consent was obtained for experiments with human subjects. Tissue samples were fixed in 4% paraformaldehyde, embedded in paraffin and processed as 5-μm thick sections.

The instrument software was Analyst QS, and the data  analysis software was BioAnalyst 1.1 . The seminiferous tubule extract was used for immunoprecipitation, and the sperm extract was used for Western blotting and peroxidase enzymatic activity estimation. Primary antibodies for non-mammalian targets are useful in detecting proteins of plant, viral, bacterial, avian, fish, yeast and invertebrates. Some examples include Drosophila, zebrafish, Xenopus, Arabidopsis, C.
Thus, the mammalian expression vector encoding GFP-PRDX2 and PRDX2 siRNA are useful tools to explore the role of PRDX2 in oxidative stress-mediated cell death in HCC SMMC-7721 cells. TNF-α usually does not induce cell death unless de novo protein synthesis is blocked . In this regard, protein synthesis inhibitor cloheximide is widely used to facilitate TNF-α-induced cell death, which includes both apoptosis and necrosis .
Related pseudogenes have been identified on chromosomes 5, 6, 10 and 13. Hwang et al. found that 5-FU treatment induced ROS generation in human lung carcinoma cells, leading to a higher level of antioxidant enzymes and resistance to 5-FU.  PRDX proteins protect MCF-7 breast cancer cells from doxorubicin-induced toxicity .

The tumor suppressor p53 also binds to HIF-1α and induces MDM2-dependent ubiquitination and proteasomal degradation of HIF-1α . Finally, HIF-1α is also subject to lysosomal degradation through chaperone-mediated autophagy, which is mediated by binding of HSC70 and LAMP2A . Moon JC, Hah YS, Kim WY, Jung BG, Jang HH, Lee JR, Kim SY, Lee YM, Jeon MG, Kim CW, et al. Oxidative stress-dependent structural and functional switching of a human 2-Cys peroxiredoxin isotype II that enhances HeLa cell resistance to H2O2-induced cell death. O'Neill et al. showed that nontranscriptional mechanisms are sufficient to sustain circadian timekeeping in the eukaryotic lineage, although they normally function in conjunction with transcriptional components. They identified oxidation of peroxiredoxin proteins as a transcription-independent rhythmic biomarker, which is also rhythmic in mammals.
Affinity purified immunoglobulins —conjugated and unconjugated—are available as negative controls for flow cytometry , immunofluorescence , immunohistochemistry , immunoprecipitation and Western Blotting applications. Mouse secondary antibodies, control sera and control immunoglobulins are also offered to be used in combination with our primary monoclonal antibodies. Western blot analysis of PRDX2 on different lysates with Mouse anti-PRDX2 antibody  (EM ) at 1/5,000 dilution. Agrawal-Singh S, Isken F, Agelopoulos K, Klein HU, Thoennissen NH, Koehler G, Hascher A, Bäumer N, Berdel WE, Thiede C, et al. Genome-wide analysis of histone H3 acetylation patterns in AML identifies PRDX2 as an epigenetically silenced tumor suppressor gene. Suspicious activity from your network has been detected by one of our administrators.